• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[用二维薄层色谱法和荧光分光光度法测定卵巢中孕酮和Δ4-孕烯-20α-醇-3-酮的含量]

[Determination of progesterone and delta 4-pregnen-20 alpha-ol-3-one contents in ovaries with two-dimensional thin-layer chromatography and spectrofluorimetry].

作者信息

Reznikov A G

出版信息

Ukr Biokhim Zh (1978). 1978 May-Jun;50(3):372-6.

PMID:664052
Abstract

The method is developed for quantitative determination of progesterone and delta4-pregnen-20alpha-ol-3-one in ovaries. It includes treatment of the gland homogenate with NaOH, ethylacetate extraction, two-dimensional thin-layer chromatography on silicaged plate Silufol UV254 in benzol-ethylacetate (80:20) and chlorophorm-ethanol (98:2) and the subsequent spectrofluorimetry. For identification of the isolated steroids their chromatographical motility, light absorption, color reactions and fluorescence spectra were studied. The contents of progesterone and delta4-pregnen-20alpha-ol-3 one in the rat ovaries were determined by this method.

摘要

该方法用于定量测定卵巢中的孕酮和4-孕烯-20α-醇-3-酮。它包括用氢氧化钠处理腺体匀浆、乙酸乙酯萃取、在硅胶板Silufol UV254上以苯-乙酸乙酯（80:20）和氯仿-乙醇（98:2）进行二维薄层色谱分析以及随后的荧光分光光度法。为鉴定分离出的类固醇，研究了它们的色谱迁移率、光吸收、颜色反应和荧光光谱。用该方法测定了大鼠卵巢中孕酮和4-孕烯-20α-醇-3-酮的含量。

相似文献

1
[Determination of progesterone and delta 4-pregnen-20 alpha-ol-3-one contents in ovaries with two-dimensional thin-layer chromatography and spectrofluorimetry].[用二维薄层色谱法和荧光分光光度法测定卵巢中孕酮和Δ4-孕烯-20α-醇-3-酮的含量]
Ukr Biokhim Zh (1978). 1978 May-Jun;50(3):372-6.
2
Microfluorometric determination of 4-pregnen-20alpha-ol-3-one separated from rat ovarian venous blood and ovary by divided thin layer chromatography.通过分溶薄层色谱法对从大鼠卵巢静脉血和卵巢中分离出的4-孕烯-20α-醇-3-酮进行微量荧光测定。
Endocrinol Jpn. 1969 Feb;16(1):55-68. doi: 10.1507/endocrj1954.16.55.
3
Isolation of 4-pregnen-20-alpha-one from rats' ovarian venous blood and ovaries.从大鼠卵巢静脉血和卵巢中分离出4-孕烯-20-α-酮。
Perspect Biol Med. 1968 Spring;11(3):427-40.
4
[Progesterone, delta-4-pregnen-20alpha-ol-3-one, delta 4-pregnen-20beta-ol-3-one and 17alpha-hydroxy-progesterone in the plasma of the umbilical vein and the umbilical artery].
Klin Wochenschr. 1962 May 1;40:453-6. doi: 10.1007/BF01737053.
5
The metabolism of progesterone to delta4-pregnen-20alpha-ol-3-one in eviscerated female rats.
J Biol Chem. 1956 Jul;221(1):461-7.
6
In vitro synthesis of 17,20 beta,21-trihydroxy-4-pregnen-3-one by ovaries of turbot (Scophthalmus maximus L.) during oocyte maturation.大菱鲆（Scophthalmus maximus L.）卵巢在卵母细胞成熟过程中体外合成17,20β,21-三羟基-4-孕烯-3-酮。
Gen Comp Endocrinol. 1997 Jul;107(1):63-73. doi: 10.1006/gcen.1997.6895.
7
[Biosynthesis of steroid hormones in human ovaries in various phases of the cycle].
Endokrinologie. 1969;55(3):145-61.
8
[Effect of uterine prostaglandin F2 alpha on the level of ovarian steroids in rats (author's transl)].子宫前列腺素F2α对大鼠卵巢甾体激素水平的影响（作者译）
Nihon Yakurigaku Zasshi. 1980 Mar;76(2):93-7.
9
Thin-layer chromatography--the forgotten alternative for the quantitative determination of steroids.薄层色谱法——类固醇定量测定中被遗忘的替代方法。
Biomed Chromatogr. 1987;2(5):189-92. doi: 10.1002/bmc.1130020503.
10
Gas chromatographic determination of unconjugated dehydroepiandrosterone, androstenedione, testosterone, pregnenolone and progesterone in human ovarian tissue.气相色谱法测定人卵巢组织中游离脱氢表雄酮、雄烯二酮、睾酮、孕烯醇酮和孕酮
Endokrinologie. 1975 Jan;64(2):151-8.