Rodgers R J, O'Shea J D, Findlay J K, Flint A P, Sheldrick E L
Endocrinology. 1983 Dec;113(6):2302-4. doi: 10.1210/endo-113-6-2302.
To determine the cellular origin of oxytocin produced by the cyclical corpus luteum (CL) of the sheep, enriched fractions of enzymatically dispersed small and large luteal cells from 12 CL were prepared on a Ficoll 400 gradient. Oxytocin was measured by RIA. Large luteal cells contained 1.08 +/- (SD) 0.70 fg/cell oxytocin, which was congruent to 30 X the content of small luteal cells. Endothelial cells contained little if any oxytocin. During a 12-h incubation, large luteal cells produced 0.28 fg/cell.h oxytocin: small luteal cells did not produce measurable amounts of oxytocin. It is concluded that the large luteal cells are the source of the oxytocin produced by the CL of the sheep.
为确定绵羊周期性黄体（CL）产生的催产素的细胞来源，在Ficoll 400梯度上制备了来自12个黄体的酶分散的小黄体细胞和大黄体细胞的富集级分。通过放射免疫分析法测定催产素。大黄体细胞含有1.08±（标准差）0.70 fg/细胞的催产素，这相当于小黄体细胞含量的30倍。内皮细胞即使有也只含有少量催产素。在12小时的孵育过程中，大黄体细胞产生0.28 fg/细胞·小时的催产素：小黄体细胞未产生可测量量的催产素。结论是大黄体细胞是绵羊黄体产生的催产素的来源。
