Sonnenfeld G, Streips U N, Costa M
Environ Res. 1983 Dec;32(2):474-9. doi: 10.1016/0013-9351(83)90127-5.
Mouse embryo fibroblast cultures were exposed to various concentrations of amorphous or crystalline NiS (nickel sulfide). After removal of the NiS from the cultures, polyriboinosinic-polyribocytidylic acid was added to induce alpha/beta interferon. Pretreatment of the cultures with crystalline NiS significantly inhibited the production of alpha/beta interferon at relatively noncytotoxic concentrations (i.e., 1 microgram/ml) while amorphous NiS had no significant effect on alpha/beta interferon production at 10 micrograms/ml. Treatment of cells with reduced glutathione did not block the inhibitory effects of crystalline NiS on alpha/beta interferon production, suggesting that these effects are not mediated by a free-radical mechanism.
将小鼠胚胎成纤维细胞培养物暴露于不同浓度的无定形或结晶态硫化镍（NiS）中。从培养物中去除NiS后，添加聚肌苷酸-聚胞苷酸以诱导α/β干扰素的产生。在相对无细胞毒性的浓度（即1微克/毫升）下，用结晶态NiS对培养物进行预处理可显著抑制α/β干扰素的产生，而无定形NiS在10微克/毫升时对α/β干扰素的产生没有显著影响。用还原型谷胱甘肽处理细胞并没有阻断结晶态NiS对α/β干扰素产生的抑制作用，这表明这些作用不是由自由基机制介导的。
