海胆精子激活的31P-NMR分析。细胞内pH值变化导致高能磷酸化合物的可逆形成。

31P-NMR analysis of sea urchin sperm activation. Reversible formation of high energy phosphate compounds by changes in intracellular pH.

作者信息

Christen R, Schackmann R W, Dahlquist F W, Shapiro B M

出版信息

Exp Cell Res. 1983 Nov;149(1):289-94. doi: 10.1016/0014-4827(83)90400-7.

Abstract

31P-NMR has been used to estimate the internal pH (pHi) of sperm from the sea urchin Strongylocentrotus purpuratus. The values for pHi obtained from the chemical shift of inorganic phosphate agree well with those obtained from amine accumulation. At low pHi, when sperm are quiescent (immotile and non-respiring), they accumulate phosphocreatine (PCr), but have a low level of inorganic phosphate (Pi). Conversely, when the pHi is elevated, sperm respiration and motility are activated, PCr is decreased and Pi is increased. This change is reversible upon decrease of the pHi, whereupon respiration and motility are arrested, Pi disappears and PCr increases. We conclude that the overall balance of energy metabolism, and thus the phosphate potential, of sea urchin sperm are under the control of the pHi.

摘要

31P-核磁共振已被用于估算紫海胆精子的内部pH值（pHi）。通过无机磷酸盐化学位移获得的pHi值与通过胺积累获得的值非常吻合。在低pHi时，精子静止（不游动且不呼吸），它们积累磷酸肌酸（PCr），但无机磷酸盐（Pi）水平较低。相反，当pHi升高时，精子呼吸和游动被激活，PCr减少而Pi增加。当pHi降低时，这种变化是可逆的，此时呼吸和游动停止，Pi消失而PCr增加。我们得出结论，海胆精子能量代谢的整体平衡，以及由此产生的磷酸盐势，受pHi的控制。