Tombes R M, Shapiro B M
Cell. 1985 May;41(1):325-34. doi: 10.1016/0092-8674(85)90085-6.
Energy utilization by the flagellum of motile sea urchin sperm is tightly coupled to the rate of energy production by the mitochondrion. This tight coupling depends upon the transport of high energy phosphate (P) from mitochondrion to axoneme, which we propose to be mediated by a phosphorylcreatine shuttle. The shuttle employs distinct mitochondrial and axonemal creatine kinase isozymes, the latter being a novel creatine kinase of 145 kd. To examine whether P is directed to the tail by such a shuttle, we inactivated creatine kinase specifically with fluorodinitrobenzene. Creatine kinase inactivation led to an inhibition of coupled, but not uncoupled, respiration and affected the pattern of sperm motility as predicted for the disruption of an obligatory link in P transport.
活动海胆精子鞭毛的能量利用与线粒体的能量产生速率紧密相关。这种紧密关联依赖于高能磷酸（P）从线粒体到轴丝的转运，我们认为这是由磷酸肌酸穿梭介导的。该穿梭利用不同的线粒体和轴丝肌酸激酶同工酶，后者是一种145kd的新型肌酸激酶。为了检验P是否通过这样的穿梭被导向尾部，我们用氟二硝基苯特异性地使肌酸激酶失活。肌酸激酶失活导致耦合呼吸（而非解偶联呼吸）受到抑制，并影响了精子运动模式，正如P转运中一个必需环节被破坏所预测的那样。
