Tombes R M, Brokaw C J, Shapiro B M
Biophys J. 1987 Jul;52(1):75-86. doi: 10.1016/S0006-3495(87)83190-9.
The significance of a phosphocreatine (PCr) shuttle in the energy transport of motile spermatozoa (Tombes, R. M., and B. M. Shapiro, 1985, Cell, 41:325-334) has been tested by a quantitative analysis of motility. Computer-assisted analysis of stroboscopic photomicrographs of live sea urchin spermatozoa whose creatine kinase has been specifically inhibited by fluorodinitrobenzene reveals that motility is impaired due to a progressive damping of bending waves as they propagate along the flagellum. This lesion, which has been defined as attenuation and can be quantified, is repaired when these spermatozoa are demembranated and reactivated to swim with ATP. The implication that attenuation is due to the inhibition of energy transport via a PCr shuttle resulting in the decrease of ATP and accumulation of inhibitory levels of ADP distally has been supported by calculating sperm PCr and ATP levels resulting from diffusion along the flagellum. The specific alterations of motility seen with creatine kinase inhibition and their reversal with ATP are as expected from the model and provide strong support for the PCr shuttle in high energy phosphate transport.
已通过对活力的定量分析来检验磷酸肌酸（PCr）穿梭在活动精子能量转运中的意义（Tombes，R.M.，和B.M. Shapiro，1985年，《细胞》，41:325 - 334）。对活海胆精子频闪显微照片进行计算机辅助分析，这些精子的肌酸激酶已被氟二硝基苯特异性抑制，结果显示活力受损是由于弯曲波沿鞭毛传播时逐渐衰减。这种病变被定义为衰减且可定量，当这些精子去膜并重新用ATP激活使其游动时，衰减得以修复。通过计算沿鞭毛扩散产生的精子PCr和ATP水平，支持了衰减是由于通过PCr穿梭的能量转运受到抑制，导致ATP减少以及远端ADP积累至抑制水平这一观点。肌酸激酶抑制时观察到的活力特定改变以及ATP使其恢复，正如该模型所预期，为PCr穿梭在高能磷酸盐转运中的作用提供了有力支持。
