Fuchs B B, Sterlina A G, Spirande I V, Zedginidze M S, Kogarko Y N
Folia Biol (Praha). 1983;29(5):358-71.
Cells of intraperitoneally transplanted murine leukaemias and sarcomas (L-1210, EL-4, MC-11 and SA-1), were found to be toxic for target cells usually employed for assaying NK cells (K-562, EL-4, YAC-1). The 3H-uridine-ribonuclease method used in our study, in contrast to the 51Cr assay, revealed not only cytotoxicity but also reversible damage to target cell membrane (membrane toxicity). This damage became irreversible if target cells had been pretreated with actinomycin D. To exclude the possible role of an admixture of NK, macrophages and T killer cells, contaminants were removed from suspensions of peritoneal tumour cells from syngeneic mice by adherence to plastic surfaces and separation on Hypaque-Ficoll, and from tumour cells grown in vivo or in vitro and then maintained in allogeneic animals by additional treatment with anti-H-2 sera and complement. The toxic effect depended directly on the dosage of tumour cells. Unlabelled target cells inhibited tumour cell toxicity. The medium used for incubating tumour cells was not toxic for target cells.
腹腔内移植的小鼠白血病和肉瘤（L-1210、EL-4、MC-11和SA-1）细胞，被发现对通常用于检测自然杀伤细胞（NK细胞）的靶细胞（K-562、EL-4、YAC-1）具有毒性。与51Cr检测法相比，我们研究中使用的3H-尿苷-核糖核酸酶法不仅揭示了细胞毒性，还揭示了对靶细胞膜的可逆性损伤（膜毒性）。如果靶细胞先用放线菌素D预处理，这种损伤就会变成不可逆的。为了排除NK细胞、巨噬细胞和T杀伤细胞混合物可能起的作用，通过贴附于塑料表面并在聚蔗糖-泛影葡胺上分离，从同基因小鼠的腹膜肿瘤细胞悬液中去除污染物，对于体内或体外生长然后在同种异体动物中维持的肿瘤细胞，则通过用抗H-2血清和补体进行额外处理来去除污染物。毒性作用直接取决于肿瘤细胞的剂量。未标记的靶细胞可抑制肿瘤细胞毒性。用于培养肿瘤细胞的培养基对靶细胞无毒。
