Kitto G B
Isozymes Curr Top Biol Med Res. 1983;11:197-211.
Isozyme techniques have proven particularly useful in the past in the field of biological insect control. In this brief review I have tried to give a small selection of the varied approaches that have been used. In the future, isozyme analysis will undoubtedly play a major role. But genetic analyses, as exemplified by the use of isozymes, form but a small part of the knowledge we must have if biological control programs are to be successful. In a pest management program where one is using both chemical and biological methods it is necessary to know a great deal about the biology of the pests, their parasites, predators, and host plants or animals, together with a knowledge of the general ecology of the area where the problem exists. Too often, major control programs have been started with pitifully inadequate basic knowledge of the insect pest concerned, dooming such projects to a series of frantic and generally makeshift attempts to redeem these inadequacies. We are starting to see a much greater emphasis on interrelationships between scientific disciplines, more basic research being conducted, and a resurgence of interaction between those people who are in the applied field and those in the basic sciences. This dialog must be continued. In closing, I want to emphasize again that in pest control we are involved in the management of a coexistence with insects and I think it appropriate to end with the thoughts of W.J. Holland [1949]: when all cities shall have been long dead and crumbled into dust, and all life shall be on the very last verge of extinction on this globe; then on a bit of lichen, growing on the bald rocks beside the eternal snows of Panama, shall be seated a tiny insect, preening its antenna in the glow of the worn-out sun, representing the sole survival of animal life on this our earth--a melancholy 'bug'.
同工酶技术过去在生物防治昆虫领域已被证明特别有用。在这篇简短的综述中,我试图对已采用的各种方法做一小部分介绍。未来,同工酶分析无疑将发挥重要作用。但是,以同工酶的使用为例的遗传分析,只是我们若要使生物防治计划取得成功所必须掌握的知识的一小部分。在一个同时使用化学和生物方法的害虫管理计划中,有必要深入了解害虫及其寄生虫、捕食者以及寄主植物或动物的生物学特性,同时还要了解问题存在地区的一般生态学知识。很多时候,重大防治计划在启动时对相关害虫的基础知识了解极其不足,这注定这些项目要进行一系列疯狂且通常是临时拼凑的尝试来弥补这些不足。我们开始看到对各学科之间相互关系的重视程度大大提高,开展了更多的基础研究,应用领域的人员与基础科学领域的人员之间的互动也再度兴起。这种对话必须持续下去。最后,我想再次强调,在害虫防治中,我们参与的是与昆虫共存的管理工作,我认为以W.J. 霍兰德[1949年]的观点作为结尾是合适的:当所有城市都早已消亡并化为尘土,地球上所有生命都濒临灭绝之际;那时,在巴拿马永恒积雪旁光秃岩石上生长的一片地衣上,会坐着一只小昆虫,在落日的余晖中梳理着它的触角,代表着我们地球上动物生命的唯一幸存者——一只忧郁的“虫子”。