Ben-Zeev O, Lusis A J, LeBoeuf R C, Nikazy J, Schotz M C
J Biol Chem. 1983 Nov 25;258(22):13632-6.
The relationship between the genes controlling heart and adipose lipoprotein lipase in fasted animals has been studied. 32 inbred mouse strains were tested for variations in heart or adipose specific activity and thermolability. The survey revealed that specific activity of heart and adipose lipoprotein lipase varied as much as 3-fold and 20-fold, respectively. In thermolability, up to a 2-fold variation was observed in the lipase in each tissue. The correlation coefficient between variations in heart and adipose lipase was apparently not significant for both parameters studied. Additional studies were performed in two strains, BALB/c and C57BL/6, along with the recombinant inbred set derived from them. The two strains did not show genetic variation for lipoprotein lipase thermolability, although the inactivation rate of heart lipase was higher than that of adipose lipase. However, BALB/c and C57BL/6 displayed significant differences in their levels of lipoprotein lipase specific activity. Thus, strain C57BL/6 showed higher heart activity when compared to BALB/c, whereas the latter showed higher adipose lipase activity when compared to C57BL/6, i.e. an inverse relationship. The specific activity levels of heart and adipose lipoprotein lipase in the recombinant inbred strains derived from BALB/c and C57BL/6 exhibited independent inheritance. Thus, in adipose tissue, a single major gene seems to control the variation observed, while the inheritance pattern of heart activity could imply involvement of more than one gene. Moreover, two out of the seven recombinant strains showed distinct recombinant phenotypes, indicating that separate unlinked genes control the variations found in heart and adipose activity. We conclude that the expression of heart and adipose lipoprotein lipase activity is under independent genetic control.
对禁食动物中控制心脏和脂肪组织脂蛋白脂肪酶的基因之间的关系进行了研究。对32个近交系小鼠品系进行了心脏或脂肪组织比活性及热稳定性变化的检测。调查显示，心脏和脂肪组织脂蛋白脂肪酶的比活性分别有高达3倍和20倍的变化。在热稳定性方面，每个组织中的脂肪酶观察到高达2倍的变化。所研究的两个参数中，心脏和脂肪组织脂肪酶变化之间的相关系数显然不显著。在BALB/c和C57BL/6这两个品系以及由它们衍生的重组近交系中进行了进一步研究。尽管心脏脂肪酶的失活率高于脂肪组织脂肪酶，但这两个品系在脂蛋白脂肪酶热稳定性方面未显示出遗传变异。然而，BALB/c和C57BL/6在脂蛋白脂肪酶比活性水平上存在显著差异。因此，与BALB/c相比，C57BL/6品系显示出更高的心脏活性，而与C57BL/6相比，后者显示出更高的脂肪组织脂肪酶活性，即呈反比关系。源自BALB/c和C57BL/6的重组近交系中，心脏和脂肪组织脂蛋白脂肪酶的比活性水平表现出独立遗传。因此，在脂肪组织中，单个主要基因似乎控制着所观察到的变异，而心脏活性的遗传模式可能意味着有多个基因参与。此外，七个重组品系中有两个显示出独特的重组表型，表明独立的非连锁基因控制着心脏和脂肪组织活性中发现的变异。我们得出结论，心脏和脂肪组织脂蛋白脂肪酶活性的表达受独立的遗传控制。
