O'Driscoll B R, Lee T H, Cromwell O, Kay A B
J Allergy Clin Immunol. 1983 Dec;72(6):695-701. doi: 10.1016/0091-6749(83)90632-2.
The release of heat-stable neutrophil chemotactic activity (NCA) has been detected after challenge of isolated human lung tissue with anti-IgE. The major NCA released (NCAL) had similar physicochemical properties to the NCA detected in the circulation of asthmatic subjects after bronchial challenge with specific antigen (NCAAG). NCAL and NCAAG (1) had molecular weights of approximately 600,000 daltons as estimated by Sephacryl S-300 gel-filtration chromatography; (2) both eluted from DEAE-Sephacel (pH 7.8) between 0.1 and 0.2 molar NaCl; (3) had isoelectric points of between 6.5 and 6.8 as determined by chromatofocusing on Polybuffer Exchanger 94. In contrast to NCAAG, lung-derived neutrophil chemotactic activity appeared to be more heterogeneous after gel-filtration and anion-exchange chromatography. The release of NCA was complete by 15 min and there was no evidence of further release up to 12 hr. These observations indicate that high-molecular-weight NCA released from human lung tissue has similar properties to NCAAG and would support the view that NCAAG originates from lung tissue after antigen bronchial challenge in asthmatic subjects.
用抗IgE刺激分离的人肺组织后，已检测到热稳定的中性粒细胞趋化活性（NCA）的释放。释放的主要NCA（NCAL）的理化性质与支气管激发特异性抗原后在哮喘患者循环中检测到的NCA（NCAAG）相似。NCAL和NCAAG：（1）通过Sephacryl S - 300凝胶过滤色谱法估计分子量约为600,000道尔顿；（2）在DEAE - Sephacel（pH 7.8）上均在0.1至0.2摩尔NaCl之间洗脱；（3）通过在Polybuffer Exchanger 94上进行聚焦色谱法测定，其等电点在6.5至6.8之间。与NCAAG相反，经凝胶过滤和阴离子交换色谱分析后，肺源性中性粒细胞趋化活性似乎更具异质性。NCA的释放在15分钟时完成，直至12小时均无进一步释放的迹象。这些观察结果表明，人肺组织释放的高分子量NCA与NCAAG具有相似的性质，并支持这样一种观点，即NCAAG起源于哮喘患者抗原支气管激发后的肺组织。
