Owhashi M, Horii Y, Ishii A
Int Arch Allergy Appl Immunol. 1985;78(4):415-20. doi: 10.1159/000233924.
Neutrophil stimulating factor (NSF), which can stimulate polymorphonuclear neutrophils (PMNs) to release eosinophil chemotactic factor (ECF), was isolated from Schistosoma japonicum soluble egg extract (SEA). The release of ECF from PMNs began as early as 5 min after stimulation and reached a peak at 40 min, and was dependent on the concentration of SEA. After Sephadex G150 gel filtration, toluene extraction, Sephadex G25 gel filtration and Dowex 1 X 8 anion exchange chromatography, NSF was identified as a hydrophilic and negatively charged component with a molecular weight of about 1,000 daltons. It was heat-stable at 100 degrees C for 60 min. NSF was easily separable from SEA-derived neutrophil chemotactic factor or from SEA-derived ECF. PMNs are suggested to be one of the sources of ECF in the eosinophil accumulation of granulomatous lesions around the deposited eggs in schistosomiasis japonica.
嗜中性粒细胞刺激因子（NSF）可刺激多形核中性粒细胞（PMN）释放嗜酸性粒细胞趋化因子（ECF），它是从日本血吸虫可溶性虫卵提取物（SEA）中分离出来的。PMN释放ECF早在刺激后5分钟就开始了，并在40分钟时达到峰值，且依赖于SEA的浓度。经过Sephadex G150凝胶过滤、甲苯萃取、Sephadex G25凝胶过滤和Dowex 1 X 8阴离子交换色谱法后，NSF被鉴定为一种亲水性带负电荷的成分，分子量约为1000道尔顿。它在100℃下60分钟内热稳定。NSF很容易与SEA衍生的嗜中性粒细胞趋化因子或SEA衍生的ECF分离。在日本血吸虫病中，沉积虫卵周围肉芽肿病变的嗜酸性粒细胞积聚过程中，PMN被认为是ECF的来源之一。
