Jose P J, Forrest M J, Williams T J
J Exp Med. 1983 Dec 1;158(6):2177-82. doi: 10.1084/jem.158.6.2177.
We describe a radioimmunoassay for rabbit C5a and its use to obtain evidence of extravascular C5a generation in two inflammatory reactions in the peritoneal cavity. These observations, together with the potent activity of C5a in inducing increased microvascular permeability involving circulating PMN leukocytes, strengthen the case for considering C5a an important inflammatory mediator. These findings offer an explanation for the many different experimental inflammatory reactions where oedema formation can be suppressed either by systemic depletion of complement or by depletion of circulating PMN leukocytes.
我们描述了一种针对兔C5a的放射免疫分析方法,并利用该方法获得了腹腔内两种炎症反应中血管外C5a生成的证据。这些观察结果,连同C5a在诱导涉及循环PMN白细胞的微血管通透性增加方面的强大活性,进一步证明了将C5a视为一种重要炎症介质的合理性。这些发现为许多不同的实验性炎症反应提供了解释,在这些反应中,水肿形成可通过全身性补体耗竭或循环PMN白细胞耗竭来抑制。