Flerov M A
Vopr Med Khim. 1978 Mar-Apr;24(2):174-80.
Ratio and intensity of metabolism of phospholipid separate fractions were studied in neurons and neuroglia, isolated from brain cortex by differential ultracentrifugation in density gradient of sucrose and Ficoll with the rate of enriching up to 90% and 80%, respectively. Neurons were characterized by the higher ratio of monophosphoinositides and lysophosphatidyl cholines as compared with neuroglia. Content of phosphatide acids and sphingomyelins was higher in neuroglia. The most specific radioactivity was observed in phosphatide acids both in neurons and neuroglia if 2(-14)C-acetate was administered. Neurons were shown to have higher metabolism of the separate fractions of phospholipids.
通过在蔗糖和菲可密度梯度中进行差速超速离心，从大脑皮层分离出神经元和神经胶质细胞，其富集率分别高达90%和80%，研究了磷脂不同组分的代谢比率和强度。与神经胶质细胞相比，神经元的单磷酸肌醇和溶血磷脂酰胆碱的比率更高。神经胶质细胞中磷脂酸和鞘磷脂的含量更高。如果给予2(-14)C-醋酸盐，在神经元和神经胶质细胞的磷脂酸中均观察到最高的比放射性。结果表明，神经元中磷脂各组分的代谢更高。
