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2
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本文引用的文献

1
Interactions between the regulation of the intracellular pH and sodium activity of sheep cardiac Purkinje fibres.绵羊心脏浦肯野纤维细胞内pH调节与钠活性之间的相互作用。
J Physiol. 1980 Jul;304:471-88. doi: 10.1113/jphysiol.1980.sp013337.
2
Appearance of calcium action potentials in crayfish slow muscle fibres under conditions of low intracellular pH.在细胞内低pH条件下小龙虾慢肌纤维中钙动作电位的出现
J Physiol. 1980 May;302:335-46. doi: 10.1113/jphysiol.1980.sp013246.
3
The ionic mechanism of intracellular pH regulation in crayfish neurones.小龙虾神经元内pH调节的离子机制。
J Physiol. 1981 Jul;316:293-308. doi: 10.1113/jphysiol.1981.sp013788.
4
Intracellular pH.细胞内pH值
Physiol Rev. 1981 Apr;61(2):296-434. doi: 10.1152/physrev.1981.61.2.296.
5
Ungulate cardiac purkinje fibres: the influence of intracellular pH on the electrical cell-to-cell coupling.有蹄类动物心脏浦肯野纤维：细胞内pH对细胞间电耦合的影响。
J Physiol. 1982 Jul;328:87-104. doi: 10.1113/jphysiol.1982.sp014254.
6
Block of endplate channels by permeant cations in frog skeletal muscle.蛙骨骼肌中渗透性阳离子对终板通道的阻断作用。
J Gen Physiol. 1981 Dec;78(6):593-615. doi: 10.1085/jgp.78.6.593.
7
pH dependence of the acetylcholine receptor channel: a species variation.乙酰胆碱受体通道的pH依赖性：种属差异
J Gen Physiol. 1981 Jun;77(6):647-66. doi: 10.1085/jgp.77.6.647.
8
Effects of ammonium ions on endplate channels.铵离子对终板通道的影响。
J Gen Physiol. 1980 May;75(5):589-613. doi: 10.1085/jgp.75.5.589.
9
Effect of divalent cations on potassium conductance of squid axons: determination of surface charge.二价阳离子对鱿鱼轴突钾离子电导的影响：表面电荷的测定
Biophys J. 1969 Mar;9(3):447-63. doi: 10.1016/S0006-3495(69)86396-4.
10
Divalent ions and the surface potential of charged phospholipid membranes.二价离子与带电磷脂膜的表面电位
J Gen Physiol. 1971 Dec;58(6):667-87. doi: 10.1085/jgp.58.6.667.

培养的鸡肌球中细胞内氢离子对乙酰胆碱受体的阻断作用的证据。

Evidence for acetylcholine receptor blockade by intracellular hydrogen ions in cultured chick myoballs.

作者信息

Goldberg G, Lass Y

出版信息

J Physiol. 1983 Oct;343:429-37. doi: 10.1113/jphysiol.1983.sp014901.

DOI:10.1113/jphysiol.1983.sp014901
PMID:6644622
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1193928/
Abstract

Acetylcholine (ACh)-induced membrane currents were recorded in voltage-clamped myoballs in culture. The ACh sensitivity and the charge transfer per channel (Qc) were doubled in NH4+ at normal extracellular pH (pHo). Immediately after wash-out of NH4+, the ACh sensitivity and Qc undershot below the control. The recovery period of the ACh sensitivity and Qc was about 20 min. The ACh sensitivity and Qc greatly increased at alkaline pHo and decreased at acid pHo. We suggest that the intracellular H+ ions can block the ACh-induced channel. The pHo effects may be mediated by a similar (but smaller) change in the intracellular pH (pHi). Intracellular alkalinization can improve the channel function.

摘要

在培养的电压钳制肌球中记录乙酰胆碱（ACh）诱导的膜电流。在正常细胞外pH（pHo）条件下，NH4+使ACh敏感性和每个通道的电荷转移量（Qc）增加一倍。NH4+洗脱后立即出现ACh敏感性和Qc低于对照的情况。ACh敏感性和Qc的恢复期约为20分钟。在碱性pHo时ACh敏感性和Qc大大增加，在酸性pHo时降低。我们认为细胞内H+离子可阻断ACh诱导的通道。pHo效应可能由细胞内pH（pHi）的类似（但较小）变化介导。细胞内碱化可改善通道功能。