Fambrough D M, Devreotes P N
J Cell Biol. 1978 Jan;76(1):237-44. doi: 10.1083/jcb.76.1.237.
Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]alpha-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated alpha-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface A Ch receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2 h.
将组织培养中的鸡骨骼肌细胞固定，并用皂角苷处理，以便在保持超微结构的同时使[125I]α-银环蛇毒素进入细胞。碘化α-银环蛇毒素与细胞内乙酰胆碱（ACh）受体及表面ACh受体结合的动力学过程相似。大约一半的细胞内ACh受体是新合成的，且处于通向并入质膜的途径中。对这一受体群体进行的相关电子显微镜放射自显影和动力学研究表明，新合成的ACh受体中有很大一部分位于高尔基体中，它们在那里停留约2小时。
