Capo C, Farnarier C, Benoliel A M, Bongrand P, Depieds R
J Reticuloendothel Soc. 1983 Nov;34(5):359-69.
The acridine orange technique was used to explore phagosome-lysosome fusion (P-L fusion) in thioglycollate-elicited rat peritoneal macrophages. Sheep red blood cells were coated with IgG or IgM plus complement, or treated with neuraminidase, tannic acid or glutaraldehyde; then both their capacity to be ingested by macrophages and their ability to induce P-L fusion after ingestion were assayed. Their capacity to be engulfed by macrophages was similar, but glutaraldehyde-treated erythrocytes were far more efficient than the other particles in triggering P-L fusion. Hence, both processes must be driven by different mechanisms. No correlation was found between the surface charge of test particles (as assayed by cell electrophoresis) and their ability to trigger phagocytosis or P-L fusion. However, glutaraldehyde-treated erythrocytes were found to be more hydrophobic than the other particles, as previously reported. Hence, particle hydrophobicity might favor P-L fusion. The implication of these findings are discussed.
采用吖啶橙技术研究了硫代乙醇酸盐诱导的大鼠腹腔巨噬细胞中吞噬体-溶酶体融合（P-L融合）。用IgG或IgM加补体包被绵羊红细胞，或用神经氨酸酶、单宁酸或戊二醛处理；然后检测它们被巨噬细胞摄取的能力以及摄取后诱导P-L融合的能力。它们被巨噬细胞吞噬的能力相似，但戊二醛处理的红细胞在触发P-L融合方面比其他颗粒更有效。因此，这两个过程必定由不同机制驱动。未发现测试颗粒的表面电荷（通过细胞电泳测定）与其触发吞噬作用或P-L融合的能力之间存在相关性。然而，如先前报道的那样，发现戊二醛处理的红细胞比其他颗粒更疏水。因此，颗粒疏水性可能有利于P-L融合。讨论了这些发现的意义。
