[大鼠肝脏透明质部分的硫胺素及硫胺素二磷酸结合能力]
[Thiamine- and thiamine diphosphate-binding capacity of the hyaloplasmic fraction of rat liver].
作者信息
Vinogradov V V, Strumilo S A, Mandrik K A, Ostrovskiĭ Iu M
出版信息
Vopr Med Khim. 1978 May-Jun;24(3):383-9.
Hyaloplasmic fraction of rat liver tissue did not bind 14C-thiamin and 14C-thiamin diphosphate in vitro as shown by means of gel filtration and equilibrium dialysis. All the thiamin and 2/3 of thiamin diphosphate were in vivo in free state or in a state unbound with proteins. Hyaloplasm lost readily the endogenous thiamin diphosphate during dialysis. Endogenous thiamin diphosphate (about 1/3 of the initial content), resistent to dialysis and gel filtration, was apparently included in transketolase in hyaloplasmic fraction of rat liver tissue.
通过凝胶过滤和平衡透析表明，大鼠肝脏组织的透明质部分在体外不结合14C-硫胺素和14C-硫胺素二磷酸。所有硫胺素和三分之二的硫胺素二磷酸在体内处于游离状态或与蛋白质未结合的状态。透明质在透析过程中很容易失去内源性硫胺素二磷酸。内源性硫胺素二磷酸（约为初始含量的三分之一），对透析和凝胶过滤具有抗性，显然包含在大鼠肝脏组织透明质部分的转酮醇酶中。
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