Petrovskiĭ K S, Sukhanov B P, Rogozhin S V
Vopr Pitan. 1978 May-Jun(3):48-53.
Subject to determination was the biological value of protein separated from food yeast by using chemical, enzymatic (chargeability with proteolytic enzymes) and biological (growing male rattlings-weanglings with the initial mass of 45 +/- 1.0 g) methods. The protein content (with no account of nucleinic acids) in rations balanced as to all the ingredients and energy amounted to 10 per cent. The experiments lasted for 28 days. In spite of good assailability of the isolate with pepsin and trypsin and a quite satisfactorily balanced of its amino acids the anabolic effect of the compound was found to be rather low, viz. PER--1.8; BV--49.1%; NPU--40.3% and in the control (caseine)--2,1; 79.0; 60.2 per cent, respectively. This is attributed to the deficiency in compound of sulphur-containing amino acids and to a relative excess of lysine. The biological value of the isolate amounted to 71 per cent of that of caseine. The method of separating protein from the yeast biomass does not have any noticeable adverse effect on its biological value.
通过化学、酶促(对蛋白水解酶的可充性)和生物学(用初始质量为45±1.0克的雄性幼鼠进行饲养)方法从食用酵母中分离出的蛋白质的生物学价值有待确定。在所有成分和能量方面都达到平衡的日粮中,蛋白质含量(不包括核酸)为10%。实验持续了28天。尽管该分离物对胃蛋白酶和胰蛋白酶的可消化性良好,且其氨基酸组成相当令人满意,但发现该化合物的合成代谢作用相当低,即蛋白质效率比值(PER)为1.8;生物价(BV)为49.1%;净蛋白质利用率(NPU)为40.3%,而对照组(酪蛋白)的相应数值分别为2.1、79.0和60.2%。这归因于该化合物中含硫氨基酸的缺乏以及赖氨酸的相对过量。该分离物的生物学价值为酪蛋白的71%。从酵母生物质中分离蛋白质的方法对其生物学价值没有任何明显的不利影响。