Baĭkova D, Parlapanova M
Vopr Pitan. 1985 May-Jun(3):26-8.
The authors studied the effect of postponed enrichment of low-grade protein (gluten) by the limiting amino acids (lysine and threonine) on the intensity of the cellular protein synthesis and on the PER. It was established that the rate of incorporation of the labeled amino acid 14C-leucine into cellular proteins changes parallel with change in the PER. Low-grade protein nutrition has an adverse effect on the metabolism. If the diet containing low-grade protein is supplemented with amino acids, the intensity of metabolic processes rises almost to the level seen during adequate animal protein nutrition. A direct and statistically significant correlation was found between the biological value of the protein consumed and the intensity of protein biosynthesis in the cells. It is concluded that measurement of the intensity of the cellular protein synthesis in a good biological test for determination of the test protein quality.
作者研究了通过限制氨基酸(赖氨酸和苏氨酸)对低质量蛋白质(麸质)进行延迟强化,对细胞蛋白质合成强度和蛋白质效率比值(PER)的影响。结果表明,标记氨基酸¹⁴C-亮氨酸掺入细胞蛋白质的速率变化与PER的变化平行。低质量蛋白质营养对新陈代谢有不利影响。如果在含有低质量蛋白质的饮食中补充氨基酸,代谢过程的强度几乎会升至充足动物蛋白质营养时的水平。在所摄入蛋白质的生物学价值与细胞中蛋白质生物合成强度之间发现了直接且具有统计学意义的相关性。得出的结论是,细胞蛋白质合成强度的测定是确定受试蛋白质质量的良好生物学测试方法。