Faict D, De Moor P, Van Baelen H, Heyns W, Stevens E, Ceuppens J
J Steroid Biochem. 1983 Oct;19(4):1397-401. doi: 10.1016/0022-4731(83)91113-5.
Human cortisol-free transcortin was prepared from charcoal-treated serum. The major purification was achieved by affinity chromatography on an immunoadsorbent column of transcortin antibodies coupled to Sepharose 4B. A further purification on hydroxylapatite yielded pure transcortin with preserved steroid-binding activity. This preparation had no significant influence on the proliferation of human lymphocytes stimulated with phytohemagglutinin, nor did it increase the inhibition of lymphocyte proliferation by cortisol or dexamethasone.
人无皮质醇皮质素转运蛋白是从经活性炭处理的血清中制备的。主要纯化步骤是通过在与琼脂糖4B偶联的皮质素转运蛋白抗体免疫吸附柱上进行亲和层析实现的。在羟基磷灰石上进一步纯化得到具有保留类固醇结合活性的纯皮质素转运蛋白。该制剂对用植物血凝素刺激的人淋巴细胞增殖没有显著影响，也没有增加皮质醇或地塞米松对淋巴细胞增殖的抑制作用。
