抗血友病因子（AHF）冻干浓缩物中VIII因子相关蛋白的可变降解

Variable degradation of factor VIII-related protein in lyophilised concentrates of antihaemophilic factor (AHF).

作者信息

Jakab T, Pflugshaupt R, Furlan M, Beck E A

出版信息

Vox Sang. 1978 Jul-Aug;35(1-2):36-40. doi: 10.1111/j.1423-0410.1978.tb02898.x.

DOI:10.1111/j.1423-0410.1978.tb02898.x

Abstract

Factor VIII-related properties (coagulant = VIII : C, 'Willebrand' factor = VIII R : WF, antigen = VIII R : AG) are measured in a constant proportion in normal plasma and certain preparations of highly purified factor VIII (relative ratios: 0.5--1.5). We tested these activities in some commercial, lyophilised concentrates of factor VIII and found a variable increase of the ratio VIII R : AG/VIII R : WF. The relative increase of VIII R : AG, and/or loss of VIII R : WF, was attributed to variable degradation of factor VIII-related protein(s) which was directly visualized by electrophoresis on 2.75% polyacrylamide gels in the presence of sodium dodecyl sulphate.

摘要

在正常血浆和某些高纯度因子VIII制剂中，VIII因子相关特性（凝血因子=VIII:C，“血管性血友病”因子=VIII R:WF，抗原=VIII R:AG）以恒定比例测定（相对比率：0.5-1.5）。我们在一些市售的冻干因子VIII浓缩物中检测了这些活性，发现VIII R:AG/VIII R:WF的比率有不同程度的升高。VIII R:AG的相对增加和/或VIII R:WF的丢失归因于VIII因子相关蛋白的不同程度降解，这在十二烷基硫酸钠存在下通过在2.75%聚丙烯酰胺凝胶上进行电泳直接观察到。