Walton D G, Acton A B, Stich H F
Mutat Res. 1983 Nov;124(2):153-61. doi: 10.1016/0165-1218(83)90175-1.
Unscheduled DNA repair synthesis (UDS) was measured autoradiographically in HF, CHO, RTG, RTO, CH and FHM cells given a 3-h exposure to MNNG, 4NQO, NA2AAF and AFB1. All the chemicals produced a dose-response, the magnitude of which varied with the particular chemical and cell line. HF produced the greatest response, CHO less and the fish cell lines the least. The response of all fish cell lines was approximately equal for a particular chemical. A number of factors were investigated to account for the comparative differences in UDS response. The time course of repair in HF, CHO and RTG following a 3-h exposure to MNNG or 4NQO was the same. As S-phase nuclei were observed in control slides and the amount of repair following UV exposure varies HF greater than CHO greater than RTG, neither 3HTdR nor mutagen uptake is limiting. The observed results are discussed.
对HF、CHO、RTG、RTO、CH和FHM细胞给予3小时的MNNG、4NQO、NA2AAF和AFB1暴露后，通过放射自显影法测量非预定DNA修复合成（UDS）。所有化学物质均产生剂量反应，其程度因特定化学物质和细胞系而异。HF产生的反应最大，CHO次之，鱼类细胞系最小。对于特定化学物质，所有鱼类细胞系的反应大致相同。研究了许多因素以解释UDS反应中的比较差异。HF、CHO和RTG在暴露于MNNG或4NQO 3小时后的修复时间进程相同。由于在对照载玻片上观察到S期细胞核，并且紫外线暴露后的修复量变化为HF＞CHO＞RTG，因此3HTdR和诱变剂摄取均不是限制因素。对观察结果进行了讨论。
