Diemer N H, Ekström von Lubitz D K
Neuropathol Appl Neurobiol. 1983 Sep-Oct;9(5):403-14. doi: 10.1111/j.1365-2990.1983.tb00125.x.
An earlier study of the rat hippocampal stratum radiatum after transient cerebral ischaemia has shown cell membrane breaks, mainly post-synaptically, occurring as early as 20 min after an ischaemic episode. In the present study HRP was injected into the lateral ventricle 10-15 min after ischaemia and allowed to diffuse until 60 min post-ischaemia. Ultrastructural examination in the control animals, showed that HRP was localized exclusively in the extracellular space. After 10 min of transient ischaemia, HRP was not confined to the extracellular space, but was also seen in about 10% of apical dendrites. Only a very few pre-synaptic terminals showed the presence of HRP. Thus, there is evidence of early post-ischaemic membrane damage occurring in vivo in the apical dendrites of the CA-1 pyramidal cells.
一项早期对短暂性脑缺血后大鼠海马辐射层的研究表明，细胞膜破裂主要发生在突触后，早在缺血发作后20分钟就会出现。在本研究中，缺血后10 - 15分钟将辣根过氧化物酶（HRP）注入侧脑室，并使其扩散至缺血后60分钟。对照动物的超微结构检查显示，HRP仅局限于细胞外间隙。短暂缺血10分钟后，HRP不仅局限于细胞外间隙，还可见于约10%的顶树突中。只有极少数突触前终末显示有HRP存在。因此，有证据表明在体内CA-1锥体细胞的顶树突中发生了早期缺血后膜损伤。
