Kratky R G, Roach M R
Scan Electron Microsc. 1983(Pt 3):1461-6.
Previous studies on cholesterol-induced sudanophilic lesions in rabbit aortas showed that the earliest lesions were periorificial with the greatest involvement distal to branch sites. In order to determine if there is a relationship between endothelial cell morphology and the regional differences in susceptibility to atherosclerosis we compared cell morphology distal to several aortic ostia and away from ostia. We examined the en face morphology of the intimal surface. Vascular casts were made of silver stained normal aortae. The surface was micrographed by SEM. Cell outlines were entered into a computer to calculate three parameters: the cell orientation index which indicates the cell shape, the en face cell area, and the distance between centers index which gives the position of the center of area with respect to the center of a longitudinal axis drawn through the cell. Differences were found between the region distal to branch sites and the region away from branch sites. Differences were also found between the regions distal to different branch sites. The complex variation of morphology led to no conclusive relationship between endothelial cell morphology and atherogenesis in the non-cholesterol-fed rabbit.
先前关于胆固醇诱导兔主动脉嗜苏丹性病变的研究表明，最早的病变位于开口周围，分支部位远端受累最为严重。为了确定内皮细胞形态与动脉粥样硬化易感性的区域差异之间是否存在关系，我们比较了几个主动脉开口远端和远离开口处的细胞形态。我们检查了内膜表面的整体形态。用银染正常主动脉制作血管铸型。通过扫描电子显微镜对表面进行显微照相。将细胞轮廓输入计算机以计算三个参数：表示细胞形状的细胞取向指数、整体细胞面积以及给出面积中心相对于穿过细胞绘制的纵轴中心位置的中心间距指数。在分支部位远端区域与远离分支部位的区域之间发现了差异。在不同分支部位远端的区域之间也发现了差异。形态的复杂变化导致在未喂食胆固醇的兔子中，内皮细胞形态与动脉粥样硬化之间没有确凿的关系。
