Francke U, de Martinville B, Coussens L, Ullrich A
Science. 1983 Dec 16;222(4629):1248-51. doi: 10.1126/science.6648531.
Fragments of the recently cloned human gene for the beta subunit of nerve growth factor (beta-NGF) were used as hybridization probes in analyzing two sets of rodent-human somatic cell hybrids for the presence of human beta-NGF sequences. Results from the first set of hybrids assigned the human beta-NGF gene to chromosome 1 and ruled out the presence of sequences of comparable homology on any other chromosome. With the second set of hybrids, which contained seven different, but overlapping, regions of chromosome 1, the NGF locus was mapped to band 1p22.
最近克隆的神经生长因子β亚基(β-NGF)人类基因片段被用作杂交探针,分析两组啮齿动物-人类体细胞杂种,以检测人类β-NGF序列的存在情况。第一组杂种的结果将人类β-NGF基因定位于1号染色体,并排除了其他任何染色体上存在具有可比同源性序列的可能性。利用包含1号染色体七个不同但相互重叠区域的第二组杂种,将NGF基因座定位到1p22带。