Flejter W L, Watkins M, Abel K J, Chandrasekharappa S C, Weber B L, Collins F S, Glover T W
University of Michigan Human Genome Center, Ann Arbor 48109-0674.
Cytogenet Cell Genet. 1993;64(3-4):222-3. doi: 10.1159/000133581.
Somatic cell hybrid mapping panels have previously been constructed to assist in the regional assignment of anonymous DNA probes and cloned genes to human chromosome 17. While a substantial number of hybrids are available that subdivide the short arm of this chromosome and the proximal portion of its long arm into specific regions, relatively few exist with breakpoints in the distal portion of the long arm. To increase the resolution of this region, four additional human x rodent somatic cell hybrids have been constructed that include breakpoints spanning the region 17q22-->q24. Hybrid clones carrying the long-arm derivative of chromosome 17 were initially identified by fluorescence in situ hybridization. Hybrids were subsequently screened using the polymerase chain reaction with primer sets representing DNA markers previously mapped to chromosome 17. These hybrids expand the existing somatic cell hybrid panel for the distal portion of the long arm of chromosome 17.
先前已构建了体细胞杂交定位板，以帮助将匿名DNA探针和克隆基因区域定位到人类17号染色体上。虽然有大量的杂交细胞可将该染色体的短臂及其长臂的近端细分为特定区域，但长臂远端具有断点的杂交细胞相对较少。为了提高该区域的分辨率，已构建了另外四个人类-啮齿动物体细胞杂交细胞，其断点跨越17q22→q24区域。最初通过荧光原位杂交鉴定携带17号染色体长臂衍生物的杂交克隆。随后使用聚合酶链反应，用代表先前已定位到17号染色体上的DNA标记的引物组对杂交细胞进行筛选。这些杂交细胞扩展了现有的针对17号染色体长臂远端的体细胞杂交板。
