Morisaki N, Matsuoka N, Shinomiya M, Mizobuchi M, Saito Y, Kumagai A
Tohoku J Exp Med. 1983 Oct;141(2):183-90. doi: 10.1620/tjem.141.183.
Several enzyme activities involved in lipid hydrolysis (acid and neutral cholesterol esterase and lipase) or synthesis (acyl-CoA synthetase, acyl-CoA: cholesterol acyltransferase and cholinephosphotransferase) were assayed in the aortas of rats with nephrosis induced by daunomycin and of rats fed on high cholesterol diet. In nephrotic rats activities of some enzymes involved in lipid hydrolysis, but not in synthesis, were increased. On the contrary, in rats fed on high cholesterol diet, the activities of all enzymes involved in lipid synthesis were significantly increased, with some increase in those involved in lipid hydrolysis. In nephrotic rats fed on high cholesterol diet all enzyme activities were markedly increased. From the view point of accumulation of cholesterol ester (CE), the ratio of hydrolysis of CE in lysosomes to CE incorporated from the blood and the ratio of hydrolysis of CE to reesterification of free cholesterol in microsomes were considered to be important. From this point of view, nephrotic hyperlipidemia was not so atherogenic as hyperlipidemia induced by the diet. The role of serum high density lipoproteins in lipid metabolism in the aorta was discussed.
对柔红霉素诱导的肾病大鼠以及喂食高胆固醇饮食大鼠的主动脉，检测了几种参与脂质水解（酸性和中性胆固醇酯酶及脂肪酶）或合成（酰基辅酶A合成酶、酰基辅酶A：胆固醇酰基转移酶和胆碱磷酸转移酶）的酶活性。在肾病大鼠中，参与脂质水解但不参与合成的某些酶的活性增加。相反，在喂食高胆固醇饮食的大鼠中，参与脂质合成的所有酶的活性均显著增加，参与脂质水解的酶活性也有一定增加。在喂食高胆固醇饮食的肾病大鼠中，所有酶的活性均明显增加。从胆固醇酯（CE）积累的角度来看，溶酶体中CE的水解与从血液中摄取的CE的比例以及CE的水解与微粒体中游离胆固醇再酯化的比例被认为很重要。从这一角度来看，肾病性高脂血症的致动脉粥样硬化性不如饮食诱导的高脂血症。讨论了血清高密度脂蛋白在主动脉脂质代谢中的作用。
