Iusupova G I
Vopr Med Khim. 1983 Sep-Oct;29(5):93-6.
Isolation of triglyceridase from pig aorta was carried out by means of a following procedure: preparation of acetone powder, extraction of the enzyme in presence of sodium deoxycholate, ammonium sulfate fractionation, chromatography on Sepharose 6B, DEAE cellulose and cholate-Sepharose. The 1,000-fold purification of the enzyme was achieved. The purified preparation of triglyceridase was free of cholesterol esterase and proteinase activities.
制备丙酮粉，在脱氧胆酸钠存在下提取酶，硫酸铵分级分离，在琼脂糖6B、DEAE纤维素和胆酸盐-琼脂糖上进行色谱分离。该酶实现了1000倍的纯化。纯化后的甘油三酯酶制剂不含胆固醇酯酶和蛋白酶活性。
