[Differences in the thermostability of aminoacyl-mRNA-synthetases from intact and denervated rabbit muscles].

The stability of alanyl-tRNA synthetase and valyl-tRNA synthetase from the intact and denervated for 30 days rabbit skeletal muscles towards the inactivating effect of heat (42 degrees for 10 min) has been studied. The activity of the enzymes was measured in the supernatant fraction obtained by centrifugation of muscle homogenates at 105, 000 g for 1 h. The stability of both alanyl- and valyl-tRNA synthetases from the denervated m. soleus and m. gastrocnemius was shown to be considerably decreased as compared with the enzymes from the intact muscles.