[Regulation by insulin of the activity of aminoacyl-tRNA synthetases in intact and denervated rabbit muscles].

The activities of Val-tRNA synthetase and Ala-tRNA synthetase in the intact rabbit M. soleus are considerably higher than those in the lateral portion of the intact M. gastrocnemius. The rates of tRNA aminoacylation by the enzymes from both denervated muscles were levelled out 35 days after sciatic nerve section. Insulin injections or diabetes did not significantly influence the aminoacyl-tRNA formation catalyzed by aminoacyl-tRNA synthetases (ARS-ases) from intact muscles. Insulin substantially increased (while diabetes decreased) the activity of both enzymes in the denervated muscles. The results obtained show that the deprivation of striated muscles of trophic nervous influence results in an increase of their sensitivity to the effect of insulin on the activity of aminoacyl-tRNA-synthetases.