Anasuya A, Narasinga Rao B S
Biochem Med. 1983 Oct;30(2):146-56. doi: 10.1016/0006-2944(83)90081-9.
An in vitro mineralizing system using bovine achilles tendon developed by Thomas and Tomita (3) was modified to enable quantitative evaluation of mineralization. Using this modified method, the potential effect of various ions on the rate of calcium uptake from inorganic mineralization medium was measured. Of the elements tested, only silicon and fluoride accelerated calcium uptake, whereas magnesium had an inhibitory effect. The simultaneous presence of silicon and fluoride in the medium had a synergistic action on calcium uptake. Urine of stone formers showed high propensity to mineralize tendon collagen, but not the urine of non-stone formers. Total content, and concentration of silicon in urine of stone formers was significantly higher than in normal urine. Addition of silicon to non-stone formers urine enhanced its capacity to mineralize collagen in vitro. These results strongly suggest the possible involvement of silicon and fluoride in the genesis of urinary calculi in man.
托马斯和富田（3）开发的一种使用牛跟腱的体外矿化系统经过改良，以实现矿化的定量评估。采用这种改良方法，测量了各种离子对无机矿化培养基中钙摄取速率的潜在影响。在所测试的元素中，只有硅和氟能加速钙的摄取，而镁则具有抑制作用。培养基中同时存在硅和氟对钙摄取具有协同作用。结石形成者的尿液显示出矿化肌腱胶原蛋白的高度倾向，但非结石形成者的尿液则不然。结石形成者尿液中的硅总含量和浓度显著高于正常尿液。向非结石形成者的尿液中添加硅可增强其在体外矿化胶原蛋白的能力。这些结果有力地表明，硅和氟可能与人尿结石的形成有关。
