Pfenninger K H, Ellis L, Johnson M P, Friedman L B, Somlo S
Cell. 1983 Dec;35(2 Pt 1):573-84. doi: 10.1016/0092-8674(83)90191-5.
The biochemical and functional characterization of the nerve growth cone is of major interest for studies on mechanisms involved in nervous system development. We describe the isolation from fetal brain of membrane-bound fragments of nerve growth cones by density gradient fractionation. These so-called growth cone particles are highly uniform and identifiable on the basis of their organelle complement. Furthermore, they co-purify in mixing experiments with fragments of radiolabeled and light microscopically identified nerve growth cones from primary cultures. The possibility of isolating growth cone fragments in quantity renders feasible the analysis of molecular mechanisms involved in growth cone function.
神经生长锥的生化和功能特性是神经系统发育相关机制研究的主要关注点。我们描述了通过密度梯度分级分离从胎儿脑中分离神经生长锥膜结合片段的方法。这些所谓的生长锥颗粒高度均匀，并且根据其细胞器组成可以识别。此外，在混合实验中，它们与来自原代培养物的放射性标记且经光学显微镜鉴定的神经生长锥片段共同纯化。能够大量分离生长锥片段使得分析生长锥功能所涉及的分子机制成为可能。
