Mothersill C, Seymour C B, Moriarty M
Cell Biol Int Rep. 1983 Nov;7(11):971-80. doi: 10.1016/0309-1651(83)90217-5.
Treatment of mammalian cells with lactate or inhibitors of glycolysis alters their radiation response, particularly in the low dose region of the dose response curve. The occurrence of both high lactate levels and high glycolytic metabolism in tumours is well known and therefore the effect of lactate on a cell line sensitive to radiation induced transformation was examined using a single exposure to Cobalt 60 gamma rays as the carcinogen challenge. The results indicate that cells treated with 5mM lactate before irradiation exhibit changes in morphology and growth rate and that the transformation frequency is increased by three to ten fold following 24 hours lactate treatment just prior to irradiation. Examination of radiation survival curves showed a positive correlation between transformation frequency and size of the shoulder, but increasing transformation frequency was associated with a decrease in Do. A mechanism involving altered Redox potential in lactate treated cells is suggested. The results are discussed in terms of their possible significance for radiotherapy.
用乳酸或糖酵解抑制剂处理哺乳动物细胞会改变它们的辐射反应，尤其是在剂量反应曲线的低剂量区域。肿瘤中高乳酸水平和高糖酵解代谢的存在是众所周知的，因此，使用单次暴露于钴60伽马射线作为致癌挑战，研究了乳酸对辐射诱导转化敏感的细胞系的影响。结果表明，照射前用5mM乳酸处理的细胞在形态和生长速率上出现变化，并且在照射前24小时乳酸处理后，转化频率增加了三到十倍。对辐射存活曲线的检查表明，转化频率与肩部大小呈正相关，但转化频率的增加与Do的降低有关。有人提出了一种涉及乳酸处理细胞中氧化还原电位改变的机制。就其对放射治疗的可能意义对结果进行了讨论。
