Terasima T, Yasukawa M, Kimura M
Gan. 1981 Oct;72(5):762-8.
The frequency of transformation induced in plateau phase 10T 1/2 cells by a single dose of X-rays decreased as a function of time during the early post-irradiation period (0 approximately 6 hr), and then tended to increase during the late post-irradiation period (7 approximately 48 hr). The extent of reduction, as measured in terms of the ratio (6 hr/0 hr value) of transformation, ranged from 0.2 to 1.1, depending upon the batch of serum which was used for pre-irradiation culture. The above findings, together with the results of a serum-free experiment, suggested that a factor affecting such reduction is present in sera. Transformation frequency depended not only on the serum used for pre-irradiation culture but also on the serum for assay culture. To maintain internal consistence of results from a series of experiments, the use of a single batch of serum is essential.
在照射后早期（0至6小时），单剂量X射线诱导处于平台期的10T 1/2细胞发生转化的频率随时间降低，而在照射后晚期（7至48小时）则趋于升高。以转化比率（6小时/0小时的值）衡量的降低程度，根据用于照射前培养的血清批次不同，范围在0.2至1.1之间。上述发现，连同无血清实验的结果表明，血清中存在影响这种降低的因素。转化频率不仅取决于用于照射前培养的血清，还取决于用于检测培养的血清。为保持一系列实验结果的内部一致性，使用同一批次的血清至关重要。
