Hunter I, Attock B, Palmer T
Clin Chim Acta. 1983 Nov 30;135(1):73-82. doi: 10.1016/0009-8981(83)90390-x.
A method is presented for the preparation and assay of lactate dehydrogenase isoenzymes 1 and 2 as pure fractions. The total method involves the use of heat treatment to destroy the heat-labile fractions (isoenzymes 3, 4 and 5); fractionation using an ion-exchange resin; and immunochemical blocking of the muscle subunit, to remove any contamination of the fraction containing isoenzyme 1 with isoenzyme 2 and vice versa. Finally, the enzyme activities in the various fractions are measured using a standard kinetic assay procedure. Estimates can be obtained of all five isoenzymes if the heat treatment step is omitted.
本文介绍了一种制备和测定乳酸脱氢酶同工酶1和2纯组分的方法。整个方法包括利用热处理破坏热不稳定组分（同工酶3、4和5）；使用离子交换树脂进行分级分离；以及对肌肉亚基进行免疫化学封闭，以去除含有同工酶1的组分中被同工酶2污染的部分，反之亦然。最后，使用标准动力学测定程序测量各组分中的酶活性。如果省略热处理步骤，则可以获得所有五种同工酶的估计值。
