Pettit S, Nealon D A, Henderson A R
Clin Chim Acta. 1980 Jan 1;100(1):59-63. doi: 10.1016/0009-8981(80)90186-2.
We describe a simple ion-exchange chromatography technique for the rapid preparation of lactate dehydrogenase-2 from lysed human erythrocytes obtained either from plasma or serum clots. The procedure appears capable of scale-up to larger columns thus making it ideal as an initial step in a more extensive purification scheme.
我们描述了一种简单的离子交换色谱技术,用于从从血浆或血清凝块中获得的裂解人红细胞中快速制备乳酸脱氢酶-2。该方法似乎能够扩大到更大的柱,因此使其成为更广泛纯化方案中理想的第一步。