Greyling H J, Schwager S, Sewell B T, von Holt C
Eur J Biochem. 1983 Dec 1;137(1-2):221-6. doi: 10.1111/j.1432-1033.1983.tb07818.x.
Histone octamers were reconstituted from the following preparations: (a) natural histone H3-H4 tetramer and histone H2A-H2B dimer, either selectively extracted from chromatin with solutions chloride or prepared by dissociation of the natural octamer; (b) acid-denatured core histones, either an unfractionated mixture or individually purified proteins. Complexes assembled from these histones elute from exclusion chromatography columns with octamer size as verified by cross-linking with dimethylsuberimidate. The reconstituted octamers all crystallize in the same form of helical tubes as the natural octamer.
(a) 天然组蛋白H3-H4四聚体和组蛋白H2A-H2B二聚体，二者均可通过用氯化物溶液从染色质中选择性提取获得，或通过天然八聚体的解离制备；(b) 酸变性核心组蛋白，可为未分级的混合物或单独纯化的蛋白质。由这些组蛋白组装而成的复合物从排阻色谱柱上洗脱时，其大小与八聚体相同，这通过与亚胺基二甲酸酯交联得以验证。重构的八聚体均以与天然八聚体相同的螺旋管形式结晶。
