从酸提取的鸡红细胞组蛋白中重建八聚体。

Octamer reconstitution from acid-extracted chicken erythrocyte histones.

作者信息

Lindsey G G, Thompson P, Pretorius L, Purves L R, von Holt C

出版信息

FEBS Lett. 1983 May 8;155(2):301-5. doi: 10.1016/0014-5793(82)80625-x.

Abstract

Histone octamers have been reconstituted from acid-extracted chicken erythrocyte histones. By the criteria of molecular size on exclusion chromatography as well as sedimentation velocity and conformational properties established by circular dichroism, fluorescence spectroscopy and imido-ester cross-linking, the reconstituted octamers have a structure identical to that of salt-extracted octamers.

摘要

已从酸提取的鸡红细胞组蛋白中重组出组蛋白八聚体。根据排阻色谱法测定的分子大小标准，以及通过圆二色性、荧光光谱和亚胺酯交联确定的沉降速度和构象性质，重组八聚体的结构与盐提取八聚体的结构相同。