Vanhove A, Breton M, Polonovski J
Adv Exp Med Biol. 1978;101:127-34. doi: 10.1007/978-1-4615-9071-2_12.
Subfractionation of the fat free homogenate of rat adipose tissue showed that a high yield of triglyceride lipase was recovered reproducibly in the microsomal supernatant fraction (cytosol) when rat epididymal fat pads were homogenized in sucrose-EDTA-Tris medium. Triglyceride lipase was bound on heparin-Sepharose. Hydrolyzing activity towards triacylglycerol was eluted as a single, sharp peak in 0.7 M NaCl, 5 mM sodium barbital and 20% glycerol (pH 7.0). The triglyceride lipase was not inhibited by 1 M NaCl and not stimulated by the presence of fresh human serum. A lipoprotein-lipase activity was demonstrable in the cytosol when adipose tissue from fed rats were used. Fasting of the animals lowered this activity.
大鼠脂肪组织无脂匀浆的亚分级分离表明，当大鼠附睾脂肪垫在蔗糖 - 乙二胺四乙酸 - 三羟甲基氨基甲烷（sucrose-EDTA-Tris）培养基中匀浆时，微粒体上清液部分（胞质溶胶）可重复性地回收高产率的甘油三酯脂肪酶。甘油三酯脂肪酶结合在肝素 - 琼脂糖上。对三酰甘油的水解活性在0.7M氯化钠、5mM巴比妥酸钠和20%甘油（pH 7.0）中以单一尖锐峰的形式洗脱。甘油三酯脂肪酶不受1M氯化钠抑制，也不受新鲜人血清存在的刺激。当使用喂食大鼠的脂肪组织时，胞质溶胶中可证明有脂蛋白脂肪酶活性。动物禁食会降低这种活性。
