Matsumoto K, Ochiai T, Sekita K, Kawasaki Y, Yasuhara K, Furuya T
Jikken Dobutsu. 1983 Jul;32(3):115-22.
Automatic blood cell analyzer "MICROX" was examined for possible applicability to the differential count of white blood cell in the rabbit, rat and mouse. Spun blood smear slides prepared from 37 rabbits were treated with Wright-Giemsa stain, and those from 49 rats and 46 mice were stained with Wright's dye. Totals of 4272 white blood cells of rabbit, 5484 cells of rat and 5329 cells of mouse were counted, respectively. The identification rate of MICROX was checked by the cell-by-cell counting method. Analysis of the data was made to examine correlation between the results with a MICROX and those of visual counting by optical microscopy on the same slides. 1) Corrected identification rates were 98.0%, 98.2% and 97.8% respectively in rabbits, rats and mice. 2) A high degree of correlation was observed between the results by MICROX and by optical microscopy in respect of segmented neutrophils and lymphocytes. 3) There were an average of 13.2 cells counted as unknown cells in rabbit blood, 10.5 in rat blood and 13.6 in mouse blood per 100 cells, respectively. Of the unknown cells about 30 to 40% were closely disposed leukocytes within the same counting squares. Almost all of atypical lymphocytes and erythroblasts were also counted as unknown cells on the analyzer. The data support the feasibility of the use of MICROX for the differential count of white blood cells of normal rabbits, rats and mice as well as monkeys and dogs.
对自动血细胞分析仪“MICROX”进行了检测，以确定其是否可用于兔、大鼠和小鼠白细胞的分类计数。对37只兔子制备的推片血涂片用瑞氏 - 吉姆萨染色，49只大鼠和46只小鼠的血涂片用瑞氏染料染色。分别对兔的4272个白细胞、大鼠的5484个细胞和小鼠的5329个细胞进行了计数。通过逐个细胞计数法检查了MICROX的识别率。对数据进行分析，以检验MICROX的结果与同一载玻片上光学显微镜目视计数结果之间的相关性。1）兔、大鼠和小鼠的校正识别率分别为98.0%、98.2%和97.8%。2）在分叶中性粒细胞和淋巴细胞方面，观察到MICROX的结果与光学显微镜的结果之间存在高度相关性。3）每100个细胞中，兔血中平均有13.2个细胞被计为未知细胞，大鼠血中有10.5个，小鼠血中有13.6个。在未知细胞中，约30%至40%是同一计数方格内紧密排列的白细胞。分析仪上几乎所有的非典型淋巴细胞和成红细胞也被计为未知细胞。这些数据支持了使用MICROX对正常兔、大鼠、小鼠以及猴和狗的白细胞进行分类计数的可行性。
