Hagino K, Ochiai T, Matsumoto K, Sekita K, Kawasaki Y, Furuya T
Jikken Dobutsu. 1982 Oct;31(4):259-64.
Automatic blood cell analyzer "MICROX" was examined for possible applicability to the differential count of white blood cell in the monkey and in the dog. Spun blood smear slides prepared from 42 monkeys and 60 dogs were stained with Wright's dye and totals of 4971 white blood cells of the monkey and 7189 cells of the dog were counted, respectively. The identification rate of MICROX was checked by the cell by cell counting method on a monitor TV of the instrument. Analysis of the data was made to examine correlationship between the results of MICROX and of optical microscopy on the same slides. 1) Identification rates and misclassification rates were 97.0% and 3.0% respectively in monkeys and 96.4% and 3.6% in dogs. 2) A high degree of correlation was observed between the results by MICROX and by optical microscopy in respect of segmented neutrophils and lymphocytes. 3) There were an average of 15.5 cells counted as unknown cells in monkey blood and 16.5 in dog blood per 100 cells, respectively. Of the unknown cells about 40 to 50% were closely disposed leukocytes within the same counting squares and almost all of atypical lymphocytes and erythroblasts were also counted as unknown cells on the analyzer. The data support the feasibility of the use of MICROX for the differential count of white blood cells of normal monkeys and dogs.
对自动血细胞分析仪“MICROX”进行了检测，以确定其是否适用于猴和犬白细胞的分类计数。从42只猴和60只犬制备的血液涂片玻片用瑞氏染料染色，分别对4971个猴白细胞和7189个犬白细胞进行了计数。通过仪器监视器电视上的逐细胞计数方法检查了MICROX的识别率。对数据进行分析，以检验MICROX结果与同一玻片上光学显微镜结果之间的相关性。1）在猴中识别率和错误分类率分别为97.0%和3.0%，在犬中分别为96.4%和3.6%。2）在分叶中性粒细胞和淋巴细胞方面，观察到MICROX结果与光学显微镜结果之间存在高度相关性。3）每100个细胞中，猴血平均有15.5个细胞被计为未知细胞，犬血平均有16.5个。在未知细胞中，约40%至50%是同一计数方格内紧密排列的白细胞，分析仪上几乎所有非典型淋巴细胞和有核红细胞也被计为未知细胞。这些数据支持了使用MICROX对正常猴和犬白细胞进行分类计数的可行性。
