Schook W J, Andrés A, Puszkin S
FEBS Lett. 1983 Dec 12;164(2):303-6. doi: 10.1016/0014-5793(83)80306-8.
Clathrin-associated proteins purified from bovine brain exhibited an ultraviolet spectrum with absorbance maximum at 256 nm and were found to contain tightly bound nucleotide. This nucleotide was identified as AMP and/or ADP by thin-layer and high-performance liquid chromatographic analyses. The phosphorylation state of the bound nucleotide varied with storage conditions, suggesting that exchange with ATP might occur while a molar ratio of two nucleotides per protein molecule is maintained. This nucleotide binding site may play a role in the functions of clathrin-associated proteins.
从牛脑中纯化的网格蛋白相关蛋白呈现出在256nm处有最大吸光度的紫外光谱，并且发现其含有紧密结合的核苷酸。通过薄层色谱和高效液相色谱分析，该核苷酸被鉴定为AMP和/或ADP。结合核苷酸的磷酸化状态随储存条件而变化，这表明在维持每个蛋白质分子两个核苷酸的摩尔比的同时，可能会与ATP发生交换。这个核苷酸结合位点可能在网格蛋白相关蛋白的功能中发挥作用。
