Kehr J, Chavko M
J Chromatogr. 1985 Dec 13;345(2):267-73. doi: 10.1016/0378-4347(85)80164-x.
A rapid method for analysis of adenine nucleotides (AMP, ADP and ATP) in nervous tissue based on ion-paired reversed-phase column liquid chromatography under isocratic conditions is described. An optimal composition of elution buffer was 25 mM potassium phosphate and 4% triethylamine adjusted to pH 6.5 with phosphoric acid. Typical separation time did not exceed 10 min with a 10-cm long compact glass cartridge packed with 5-microns silica C18. The method was employed to determine ATP, ADP and AMP concentrations in rat brain extracts and values thus obtained were compared with those published elsewhere.
本文描述了一种基于等度条件下离子对反相柱液相色谱法快速分析神经组织中腺嘌呤核苷酸（AMP、ADP和ATP）的方法。洗脱缓冲液的最佳组成为25 mM磷酸钾和4%三乙胺，用磷酸调节至pH 6.5。使用填充有5微米硅胶C18的10厘米长紧凑型玻璃柱，典型分离时间不超过10分钟。该方法用于测定大鼠脑提取物中的ATP、ADP和AMP浓度，并将所得值与其他地方发表的值进行比较。
