用克隆的乙肝病毒（HBV）DNA感染黑猩猩后发生的乙型肝炎。
Hepatitis B after infection of a chimpanzee with cloned HBV DNA.
作者信息
Will H, Darai G, Deinhardt F, Schellekens H, Schaller H
出版信息
Dev Biol Stand. 1983;54:131-3.
PMID:6653885
Abstract
Transfection of chimpanzee liver cells in vivo or in vitro with cloned HBV DNA induced typical hepatitis B in the inoculated animal. Intravenous inoculation of a 1000-fold greater dose of the same cloned HBV DNA did not induce infection or disease. The experiment proved that cloned HBV DNA is fully functional and that the nick gap structure, proteins covalently linked to the HBV genome, or any other HBV particle associated protein are not needed for initiation of virus replication.
摘要
用克隆的乙肝病毒（HBV）DNA在体内或体外转染黑猩猩肝细胞，可在接种动物中诱发典型的乙型肝炎。静脉注射剂量比相同克隆HBV DNA大1000倍的物质，并未诱发感染或疾病。该实验证明，克隆的HBV DNA具有完整功能，病毒复制起始不需要缺口结构、与HBV基因组共价连接的蛋白质或任何其他与HBV颗粒相关的蛋白质。
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