Lazizi Y, Guillon J C, Pillot J
Dev Biol Stand. 1983;54:75-9.
Monolayer cultures were obtained from human liver explants. The cells, which extend in all directions, are trypsinized then collected by differential centrifugation and incubated at 38 degrees 5. The oldest culture is 12 month-old. Albumin and alpha 1-antitrypsin are constantly detected and measured in the supernatant by radioimmunoassay. The liver specific protein is always characterized by immunofluorescent staining on the surfaces of the cultivated cells.
单层培养物取自人肝脏外植体。这些向各个方向伸展的细胞经胰蛋白酶消化后，通过差速离心收集，并在38摄氏度5的条件下孵育。最老的培养物已有12个月。通过放射免疫测定法在上清液中持续检测和测量白蛋白和α1-抗胰蛋白酶。肝脏特异性蛋白总是通过培养细胞表面的免疫荧光染色来表征。
