Tsiquaye K N, Das P K, Zuckerman A J
Br J Exp Pathol. 1978 Oct;59(5):482-8.
Livers from human foetuses obtained from terminations of pregnancy by suction were used to establish long-term cultures of hepatocytes. Functional activities of the hepatocellular population in these cultures were monitored by immunoperoxidase staining during and up to the 90 days' life of the cultures. Intracellular localization of albumin and alpha-foetoprotien in cultures of the same age and from the same specimen indicate that syntheses of these two markers in foetal hepatocytes occur simultaneously. No qualitative differences were observed in the biosynthetic capacity of the cells maintained in tissue culture medium with and without serum. Fibroblast-like cells seen in the almost homogeneous population of hepatocytes did not stain for albumin and alpha-foetoprotein.
通过抽吸终止妊娠获得的人类胎儿肝脏被用于建立肝细胞的长期培养。在这些培养物长达90天的培养期间及之后，通过免疫过氧化物酶染色监测这些培养物中肝细胞群体的功能活性。来自相同年龄和同一样本的培养物中白蛋白和甲胎蛋白的细胞内定位表明，胎儿肝细胞中这两种标志物的合成同时发生。在含血清和不含血清的组织培养基中培养的细胞，其生物合成能力未观察到质的差异。在几乎均一的肝细胞群体中看到的成纤维细胞样细胞，白蛋白和甲胎蛋白染色均为阴性。
