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本文引用的文献

1
GROWTH OF HUMAN BREAST NEOPLASMS IN CELL CULTURE.人乳腺肿瘤在细胞培养中的生长
J Natl Cancer Inst. 1965 Feb;34:217-29. doi: 10.1093/jnci/34.2.217.
2
Cultivation of human breast carcinomas.人乳腺癌的培养
J Natl Cancer Inst. 1958 Dec;21(6):1131-47.
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Analytical study of the cells in cervical squamous-cell cancer.宫颈鳞状细胞癌中细胞的分析研究。
Lab Invest. 1957 May-Jun;6(3):241-50.
4
Unusual patterns of squamous epithelium of the uterine cervix: cytologic and pathologic study of koilocytotic atypia.子宫颈鳞状上皮的异常形态：凹空细胞异型性的细胞学与病理学研究
Ann N Y Acad Sci. 1956 Mar 30;63(6):1245-61. doi: 10.1111/j.1749-6632.1956.tb32134.x.
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The cellular morphology of carcinoma in situ and dysplasia or atypical hyperplasia of the uterine cervix.子宫颈原位癌及发育异常或非典型增生的细胞形态。
Cancer. 1953 Mar;6(2):224-34. doi: 10.1002/1097-0142(195303)6:2<224::aid-cncr2820060203>3.0.co;2-h.
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Cytoplasmic:nuclear ratios in the cytological diagnosis of cancer.细胞质与细胞核的比例在癌症细胞学诊断中的应用
Cancer. 1952 Sep;5(5):945-9. doi: 10.1002/1097-0142(195209)5:5<945::aid-cncr2820050510>3.0.co;2-9.
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Cytologic manifestations of neoplastic transformation in vitro.体外肿瘤转化的细胞学表现。
J Natl Cancer Inst. 1970 Jan;44(1):39-63.
8
Cell-to-substrate adhesions during spreading and locomotin of carcinoma cells. A study by mcrocinematography and reflection contrast microscopy.癌细胞铺展和运动过程中的细胞与底物黏附。显微电影摄影术和反射对比显微镜研究。
Exp Cell Res. 1980 Aug;128(2):249-56. doi: 10.1016/0014-4827(80)90061-0.
9
Interrelation of motility, shape, and surface architecture in human carcinoma cells.
Cell Biol Int Rep. 1981 Sep;5(9):929-36. doi: 10.1016/0309-1651(81)90208-3.
10
Nuclear feulgen DNA content and nuclear size in human breast carcinoma.人类乳腺癌中的核福尔根DNA含量与核大小
Hum Pathol. 1982 Jul;13(7):626-30. doi: 10.1016/s0046-8177(82)80004-x.

异种移植的人乳腺癌细胞的体外培养。

In vitro cultivation of xenotransplanted human breast-cancer cells.

作者信息

Volk M, Scheuffele J, Bastert G, Naujoks H

出版信息

J Cancer Res Clin Oncol. 1983;106(3):179-86. doi: 10.1007/BF00402605.

DOI:10.1007/BF00402605
PMID:6654952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12253851/
Abstract

Xenotransplanted human breast-cancer tissue growing in the second to the ninth passage in nu/nu mice and normal tissue from nu/nu mice was cultivated in vitro. Thirty-two of fifty-two (62%) cultures grew successfully and have been analyzed. The cells were observed using phase-contrast microscopy and May-Grünwald-Giemsa staining. The following cell types could be distinguished: normal fibroblast-like cells (FC), normal epithelial-like cells (EP I), abnormal epithelial-like cells (EP II), and degenerated or dying cells (DC). After staining, the nuclear area of all cell types was measured. The results were: FC = 280 micrometers 2, EP I = 320 micrometers 2, and EP II = 800 micrometers 2. They were compared with the nuclear area of human breast-cancer cells obtained by aspiration biopsy.

摘要

将在无胸腺裸鼠体内传代至第二代至第九代的人乳腺癌组织异种移植瘤以及无胸腺裸鼠的正常组织进行体外培养。52个培养物中有32个（62%）成功生长并进行了分析。使用相差显微镜和May-Grünwald-Giemsa染色观察细胞。可区分出以下细胞类型：正常成纤维样细胞（FC）、正常上皮样细胞（EP I）、异常上皮样细胞（EP II）以及退化或濒死细胞（DC）。染色后，测量所有细胞类型的核面积。结果为：FC = 280平方微米，EP I = 320平方微米，EP II = 800平方微米。将它们与通过针吸活检获得的人乳腺癌细胞核面积进行比较。