Isacke C M, Deller M J
J Cell Physiol. 1983 Dec;117(3):407-14. doi: 10.1002/jcp.1041170316.
Malignant PC13 embryonal carcinoma (EC) cells differentiate in vitro in response to retinoic acid, giving rise to a population of benign endoderm-like cells (END), termed PC13 END. PC13 EC and PC13 END cells exhibit growth cooperativity in co-culture, whereby the EC cells stimulate END cell proliferation and the END cells can support EC cell multiplication. The EC cells' stimulatory effect operates via soluble, diffusible factors which are also active on a range of fibroblast cell lines. END cells support the multiplication of EC cells plated at low density, via a multifactorial mechanism. Contact-dependent effects can operate in the absence of END cell metabolic activity, while contact-independent effects require the continuous presence of live END cells. It was observed that there was a variation in the ability of fibroblast cell lines to act as EC cell feeders. Similar interactive events may be important during the in vivo proliferation and differentiation of teratocarcinoma cells and their embryonic counterparts.
恶性PC13胚胎癌细胞在体外对视黄酸产生反应而分化，产生一群良性内胚层样细胞（END），称为PC13 END。PC13 EC细胞和PC13 END细胞在共培养中表现出生长协同性，其中EC细胞刺激END细胞增殖，而END细胞可支持EC细胞增殖。EC细胞的刺激作用通过可溶、可扩散因子发挥，这些因子对一系列成纤维细胞系也有活性。END细胞通过多因素机制支持低密度接种的EC细胞增殖。接触依赖性效应可在无END细胞代谢活性的情况下发挥作用，而接触非依赖性效应需要活的END细胞持续存在。据观察，成纤维细胞系作为EC细胞饲养层的能力存在差异。类似的相互作用事件在体内畸胎癌细胞及其胚胎对应物的增殖和分化过程中可能很重要。
