Peterson P K, Keane W F, Miller W, Freiberg M, Staub D, Verbrugh H A
J Infect Dis. 1983 Dec;148(6):1040-8. doi: 10.1093/infdis/148.6.1040.
Cells of the human mononuclear phagocyte system have recently been shown to possess cytophilic IgG molecules that promote phagocytosis of staphylococci bearing cell-wall protein A. In the present study, the possible mediation of a cytotoxic response to 51Cr-labeled sheep erythrocytes coated with protein A by cytophilic antibodies on human peritoneal macrophages was evaluated. The target cells were readily lysed by peritoneal macrophages. Cytotoxicity was blocked by pretreatment of macrophages with soluble protein or with anti-Fc F(ab')2 fragments. In contrast, cytotoxicity was not affected by cytochalasin B; this finding suggests that cytolysis is an extracellular event. Perturbation of cytophilic IgG with particle-bound protein A elicited a chemiluminescent response from peritoneal macrophages; however, experiments with scavengers of reactive oxygen species indicated that toxic oxygen radicals may not be required for cytotoxicity. The results indicate that cytophilic antibody-mediated cytotoxicity may contribute to cellular injury as well as host-defense aspects of the inflammatory process.
最近研究表明，人类单核吞噬细胞系统的细胞拥有亲细胞性IgG分子，可促进对携带细胞壁蛋白A的葡萄球菌的吞噬作用。在本研究中，评估了人类腹膜巨噬细胞上的亲细胞性抗体对包被有蛋白A的51Cr标记绵羊红细胞的细胞毒性反应的可能介导作用。靶细胞很容易被腹膜巨噬细胞裂解。用可溶性蛋白或抗Fc F(ab')2片段预处理巨噬细胞可阻断细胞毒性。相比之下，细胞松弛素B对细胞毒性没有影响；这一发现表明细胞溶解是一种细胞外事件。颗粒结合的蛋白A对亲细胞性IgG的扰动引发了腹膜巨噬细胞的化学发光反应；然而，活性氧清除剂的实验表明，细胞毒性可能不需要有毒的氧自由基。结果表明，亲细胞性抗体介导的细胞毒性可能导致细胞损伤以及炎症过程中的宿主防御。
