Mitsunaga M
Third Department of Internal Medicine, Okayama University Medical School, Japan.
Acta Med Okayama. 1987 Oct;41(5):205-14. doi: 10.18926/AMO/31754.
Antibody-dependent macrophage-mediated cytotoxicity was studied to determine the significance of cytophilic anti-thyroglobulin antibody (ATgA) present in the sera of patients with Hashimoto's thyroiditis. Effector cells were normal human monocytes or guinea-pig peritoneal exudate cells, and target cells were human thyroglobulin(Tg)-coated chicken erythrocytes. Cytotoxicity was evaluated by morphological observation and by 51Cr-releasing assay. Normal human monocytes rapidly destroyed ATgA-bound Tg-coated chicken erythrocytes by extracellular cytolysis and by phagocytosis. On the contrary, human monocytes "armed" with cytophilic ATgA destroyed Tg-coated chicken erythrocytes slowly and to a lesser extent, and only by extracellular cytolysis. When normal monocytes or peritoneal exudate cells were incubated with Tg-coated chicken erythrocytes in the presence of the sera of patients with Hashimoto's thyroiditis, phagocytosis occurred rapidly, but extracellular cytolysis developed rather slowly. These data suggest the possibility that human monocytes participate in antibody-dependent cell-mediated cytotoxicity (ADCC) in vivo, which may be an important destructive mechanism in Hashimoto's thyroiditis. It is also possible that ATgA cytophilic for monocytes render non-immune peripheral monocytes cytotoxic against Tg-bearing cells.
研究了抗体依赖性巨噬细胞介导的细胞毒性，以确定桥本甲状腺炎患者血清中存在的亲细胞性抗甲状腺球蛋白抗体（ATgA）的意义。效应细胞为正常人单核细胞或豚鼠腹腔渗出细胞，靶细胞为包被人甲状腺球蛋白（Tg）的鸡红细胞。通过形态学观察和51Cr释放试验评估细胞毒性。正常人单核细胞通过细胞外溶解和吞噬作用迅速破坏与ATgA结合的包被Tg的鸡红细胞。相反，用亲细胞性ATgA “武装” 的人单核细胞缓慢且程度较小地破坏包被Tg的鸡红细胞，且仅通过细胞外溶解作用。当正常单核细胞或腹腔渗出细胞在桥本甲状腺炎患者血清存在的情况下与包被Tg的鸡红细胞一起孵育时，吞噬作用迅速发生，但细胞外溶解作用发展相当缓慢。这些数据提示人单核细胞可能在体内参与抗体依赖性细胞介导的细胞毒性（ADCC），这可能是桥本甲状腺炎中一种重要的破坏机制。也有可能对单核细胞具有亲细胞性的ATgA使非免疫性外周单核细胞对携带Tg的细胞具有细胞毒性。
